Journal:

Article Title: IGF-2 and FLT-1/VEGF-R1 mRNA levels reveal distinctions and similarities between congenital and common infantile hemangioma

doi: 10.1203/PDR.0b013e318163a243

Figure Lengend Snippet: IGF-2 protein localized in relation to blood vessels in common and congenital hemangioma. A–C) CD31 immunostaining of frozen sections highlights blood vessels in proliferating common hemangioma (A), NICH (B) and RICH (C). Nuclei are stained blue with DAPI. D–E) Common hemangioma sections double-labeled with anti-IGF2 (green) and anti-CD31 (red). Nuclei stained with DAPI (blue). Panel D, a proliferating phase hemangioma while Panel E, involuting phase hemangioma. F) RICH double-labeled with anti-IGF2 (green) and anti-CD31 (red). G) NICH stained with anti-IGF2 (green). H) Pyogenic granuloma stained with anti-IGF2 (green) as negative control. Scale bars are 50 µm. Panel A is 200X magnification; Panels B, C, G and H, 100X magnification; Panels D–F, 300X magnification.

Article Snippet: Indirect immunofluorescence Cryosections ( 4 μm ) of the tumors (proliferating and involuting infantile hemangioma), RICH, NICH and pyogenic granulomas were fixed with cold acetone for 15 min. After washing for 5 min in PBS, and incubating with species appropriate blocking serum, the following primary antibodies were used: mouse anti-human IGF2 mAb 1:50 (R&D Systems, USA), mouse anti-human CD31 mAb 1:50 (DAKO, Carpinteria, CA, USA), goat anti-human CD31 antibody 1:50 (Santa Cruz Biotechnology Inc., CA, USA).

Techniques: Immunostaining, Staining, Labeling, Negative Control

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 1 MiR-33a-3p directly targeted IGF2. A A schematic diagram of the forecasted miR-33a-3p binding site to IGF2. B Association between miR-33a-3p and IGF2 was confirmed by dual-luciferase reporter assay. **P < 0.01 versus mimic control

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Binding Assay, Luciferase, Reporter Assay, Control

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 3 Expression of miR-33a-3p and IGF2 in osteogenic differentiation induced hBMSCs. A Alizarin red S was applied for detecting calcified cells. B Quantification of calcified cells. C Detection of ALP activity. D RT-qPCR analysis of Runx2, ALP and Osterix mRNA levels. E–G The expression of miR-33a-3p and IGF2 was assessed by western blot analysis or RT-qPCR analysis. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Control

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 2 Expression of miR-33a-3p and IGF2 in the serum of osteoporosis patients. A–B Levels of miR-33a-3p and IGF2 in the serum of osteoporosis patients were checked by RT-qPCR assay.**P < 0.01

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 4 Effects of miR-33a-3p on IGF2 levels in hBMSCs. hBMSCs were transfected with mimic control, miR-33a-3p mimic, inhibitor control or miR-33a-3p inhibitor for 48 h. A Detection of miR-33a-3p levels using RT-qPCR. B–C Expressions of IGF2 were assessed by RT-qPCR and western blot analysis. RT-qPCR analysis of miR-33a-3p (D) and IGF2 (E) mRNA levels in miR-33a-3p inhibitor or inhibitor control transfected cells. F Western blot analysis of miR-33a-3p and IGF2. **P < 0.01 versus Mimic control; ##P < 0.01 versus Inhibitor control

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Transfection, Control, Quantitative RT-PCR, Western Blot

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 5 Effects of IGF2-plasmid or miR-33a-3p mimic on IGF2 levels in hBMSCs. hBMSCs were transfected with mimic control, miR-33a-3p mimic, control-plasmid, IGF2-plasmid for 48 h. A–B Determination of IGF2 level in control-plasmid or IGF2-plasmid transfected cells by RT-qPCR and western blot assay. C–D Expressions of IGF2 were assessed by RT-qPCR and western blot analysis. **P < 0.01 versus Control-plasmid; ##P < 0.01 versus Mimic control; &&P < 0.01 versus miR-33a-3p mimic + control-plasmid

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Plasmid Preparation, Transfection, Control, Quantitative RT-PCR, Western Blot

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 7 Effects of miR-33a-3p mimic or IGF2-plasmid on hBMSCs viability and apoptosis. hBMSCs were transfected with mimic control, miR-33a-3p mimic, control-plasmid, IGF2-plasmid for 48 h. A MTT assay of cell viability. B Cell apoptosis was detected by Flow cytometry assay. C Quantification of apoptotic cells. **P < 0.01 versus mimic control; ##P < 0.01 versus miR-33a-3p mimic + control-plasmid

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Plasmid Preparation, Transfection, Control, MTT Assay, Flow Cytometry

Journal: Journal of orthopaedic surgery and research

Article Title: Aberrant expression of miR-33a-3p/IGF2 in postmenopausal osteoporosis patients and its role and mechanism in osteoporosis.

doi: 10.1186/s13018-023-03883-6

Figure Lengend Snippet: Fig. 6 Effects of miR-33a-3p mimic or IGF2-plasmid on hBMSCs calcification and ALP activity. hBMSCs were transfected with mimic control, miR-33a-3p mimic, control-plasmid, IGF2-plasmid for 48 h. RT-qPCR analysis and western blot assay of miR-33a-3p (A) and IGF2 (B–C) expression. D Calcified cells were determined by Alizarin Red S. E Quantification of calcified cells. F Evaluation of ALP activity. G mRNA levels of Runx2, ALP and Osterix were detected by RT-qPCR analysis. **P < 0.01 versus Control; ##P < 0.01 versus Induction + mimic control; &&P < 0.01 versus Induction + miR-33a-3p mimic + control-plasmid

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h to avoid nonspecific binding and then cultivated with primary antibodies against IGF2 (cat. no. A2086, ABclonal; 1:1000 dilution) or β-actin (cat. no. TDY051; Beijing TDY Biotech CO., LTD.; 1:1000 dilution) at 4 °C overnight.

Techniques: Plasmid Preparation, Activity Assay, Transfection, Control, Quantitative RT-PCR, Western Blot, Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques:

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Immunostaining, Control, Positive Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing, Control